Diagnostic Procedures & Therapeutic Interventions
Proficiency in ultrasound-guided amniocentesis, CVS, therapeutic amniodrainage, and fetal shunt placement.

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Detailed Course Syllabus
Aseptic Technique & Needle Guidance Systems
Transducer sheath preparation, acoustic coupling, and free-hand needle trajectory.
Amniocentesis: Entry Point & Fluid Sampling
Selecting vertical fluid pockets, post-procedure assessment, and Rhogam administration.
Chorionic Villus Sampling (CVS)
Sampling chorion frondosum at 11–14 weeks with 20G/18G coaxial needles.
Therapeutic Fetal Interventions
Vesicoamniotic and pleuroamniotic shunts, fetal blood transfusion, and emergency management.
Learning Objectives
- Execute sterile continuous needle visualization in-plane and out-of-plane.
- Safely perform diagnostic genetic amniocentesis avoiding the placenta and fetus.
- Master transabdominal and transcervical chorionic villus sampling (CVS) trajectories.
- Recognize indications and technique for therapeutic serial amniodrainage and vesicocentesis.
Clinical Relevance
Mastering needle-guidance under ultrasound minimizes fetal loss rates (<0.1%) during invasive genetic diagnostic procedures.
Clinical Guidelines Aligned
- ACOG Practice Bulletin on Invasive Prenatal Diagnostic Testing
- ISUOG Guidelines: Invasive Procedures in Obstetric Ultrasound
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